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Intramuscular <t>injections</t> of viral optogenetic constructs. (A) Schematic of macaque subjects, viral constructs, and muscles injected . Blue/reverse blue lettering indicate whether a designated muscle was injected with the ChR2- or Chronos-based construct, respectively. Muscle abbreviations: TA, tibialis anterior; GN, lateral gastrocnemius; FCR, flexor carpi radialis; PT, pronator teres; ED, extensor digitorum. (B) Identification of <t>injection</t> zones . A stimulating injection needle was advanced slowly into the muscle while applying low-amplitude electrical stimulation. After observing near-maximal contractions, indicating a zone with higher density of neuromuscular junctions (NMJs), 200 μL of viral solution was injected slowly over 1 min. This process was repeated over 10 sites per muscle or muscle group.
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Intramuscular injections of viral optogenetic constructs. (A) Schematic of macaque subjects, viral constructs, and muscles injected . Blue/reverse blue lettering indicate whether a designated muscle was injected with the ChR2- or Chronos-based construct, respectively. Muscle abbreviations: TA, tibialis anterior; GN, lateral gastrocnemius; FCR, flexor carpi radialis; PT, pronator teres; ED, extensor digitorum. (B) Identification of injection zones . A stimulating injection needle was advanced slowly into the muscle while applying low-amplitude electrical stimulation. After observing near-maximal contractions, indicating a zone with higher density of neuromuscular junctions (NMJs), 200 μL of viral solution was injected slowly over 1 min. This process was repeated over 10 sites per muscle or muscle group.

Journal: Frontiers in Neuroscience

Article Title: Viral-Mediated Optogenetic Stimulation of Peripheral Motor Nerves in Non-human Primates

doi: 10.3389/fnins.2019.00759

Figure Lengend Snippet: Intramuscular injections of viral optogenetic constructs. (A) Schematic of macaque subjects, viral constructs, and muscles injected . Blue/reverse blue lettering indicate whether a designated muscle was injected with the ChR2- or Chronos-based construct, respectively. Muscle abbreviations: TA, tibialis anterior; GN, lateral gastrocnemius; FCR, flexor carpi radialis; PT, pronator teres; ED, extensor digitorum. (B) Identification of injection zones . A stimulating injection needle was advanced slowly into the muscle while applying low-amplitude electrical stimulation. After observing near-maximal contractions, indicating a zone with higher density of neuromuscular junctions (NMJs), 200 μL of viral solution was injected slowly over 1 min. This process was repeated over 10 sites per muscle or muscle group.

Article Snippet: A 30 gauge monopolar injectable needle (Technomed, Netherlands) was attached to a tuberculin syringe filled with virus solution, while a metal hub needle attached to a ground lead was inserted through the skin edge.

Techniques: Construct, Muscles, Injection